Q: How should samples be diluted?
A: Set up a pilot dilution series based on expected concentration and kit sensitivity. Serum samples are generally recommended to start at 1:2 or 1:5.
Q: What if the standard curve R² is low?
A: Check the reconstitution and serial dilution steps, avoid bubbles and cross-contamination between wells, and clean the plate bottom before reading.
Q: No color development?
A: Confirm the addition order of substrate and stop solution, incubation temperature and time, and check for residual wash buffer.
